Development of a Novel Tissue-Specific Method to Detect Cytokeratin 20-Positive Circulating Tumor Cells in Metastatic Colorectal Cancer

Sze Chuen Cesar Wong, Charles Chan Ming Lok, Chu So Shan

Abstract


Abstract: Introduction: Although many studies have shown the vast potential of circulating tumor cells (CTCs) detection in cancer diagnosis and prognosis, our understanding of their clinical significance is still far from complete. A major obstacle arises from the lack of well-established tumor or tissue-specific markers to detect CTCs by immunocytochemical staining after immunomagnetic enrichment (IE). Methods: We have established the utility of cytokeratin 20 (CK20), a gastrointestinal tract specific marker, for the specific detection and identification of colorectal cancer (CRC) CTCs. This breakthrough was successfully validated in spike-in experiments using CRC cell line models followed by a pilot study which recruited 32 metastatic CRC patients, 25 benign colorectal diseases patients and 27 normal subjects. Results: CK20-positive CTCs were detected in 90% metastatic CRC patients but not in benign colorectal diseases patients and normal subjects using this refined assay. Conclusions: These impressive results have laid the foundation for further development of CK20-positive CTCs as a promising marker in diagnosis, prognostication and treatment monitoring of metastatic CRC.


Keywords


Tissue-specific; Cytokeratin 20-positive; Circulating tumor cells; Metastatic; Colorectal cancer

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References


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DOI: http://dx.doi.org/10.30564/amor.v4i4.192

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