Resveratrol Reduced Silver Nanoparticles for Anti-tumor Therapy

Abstract

In situ reduction is one of the easiest and most effective methods to prepare noble metal nanoparticles. The study is to trans resveratrol (RES) as the reducing agent, silver nitrate (AgNO3) as the precursor of silver nanoparticles, cetyl trimethyl ammonium bromide (CTAB) as the surfactant and phase transfer catalyst, and silver based nanoparticles (RES-AgNPs) loaded with RES were prepared by in situ liquid phase reduction process. RES-AgNPs with an average particle size of (45.5±2) nm and zeta potential of 21 mV were prepared when AgNO3, RES and CTAB reacted at 1:1:0.5 (40 °C for 13h). The infrared spectrum and ultraviolet absorption spectrum showed that the RES load on the surface of AgNPs reached 0.883 mg/mL. In addition, RES-AgNPs is sensitive to hydrogen peroxide release. When the pH value of the simulated tumor microenvironment was 5.4 and H2O2 was 25 μmol/mL, the release efficiency of RES can reach 89%. In vitro antitumor effect of RES-AgNPs, which was determined by MTT experiment, showed that compared with RES, AgNPs and RES+AgNPs, the growth inhibition rate of RES-AgNPs (15μg/mL) on MCF-7 cells was increased by 62.6%, 68.2% and 55.1%, respectively. The result reflected the great synergistic killing effect of AgNPs and RES on tumor cells.