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5-aminolevulinic Acid - Mediated Photodynamic Therapy for Antimelanoma Treatment

Katie Foxall (Medical Department, Sanofi Aventis, Johannesburg, South Africa)
Alicia McMaster (Department of Pathology, Dr. Baba Saheb Ambedkar Medical College and Hospital, Government of NCT of Delhi, New Delhi, India)

Abstract

Photodynamic therapy (PDT) is a novel approach for the anti-tumor treatment of photosensitive drugs and laser activation. In the study, 5-aminolevulinic acid (ALA) was used as a photosensitizer to mediate PDT to inhibit the growth, metastasisand epithelial-mesenchymal transformation of melanoma cells. Firstly, the therapeutic effect of PDT was investigated through invitro experiments. Human melanoma A375 cells cultured with ALA were treated with different light intensity, and the cell survivalrates of A375 cells were measured by CCK8 method.Subsequently, the metastasis and invasion rates of A375 cells were detectedby scratch test and Transwell test. Western blot was used to detect the expression of molecules related to epithelial mesenchymal transformation, such as E-cadherin, Snail, TGF-β, Smad2 and Smad3. In vitro results showed that A375 cells in the ALA-PDTtreatment group showed weaker migration and invasion ability, while A375 cells in the control group showed higher migrationand invasion rate (P<0.05). E-cadherin expression was significantly increased in the ALA-PDT treatment group (P<0.01), while expression of Snail, Smad2, Smad3 and TGF-β were significantly decreased in the ALA-PDT treatment group (P<0.05). Therefore,ALA is a good photosensitizer that can inhibit the migration and invasion of melanoma, which also can inhibit the epithelial mesenchymal transformation of melanoma.

Keywords

Photodynamic therapy; Melanoma; Photosensitizer

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References

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DOI: http://dx.doi.org/10.30564/amor.v5i3.242

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