Electrified Water as a Regulator of Cell Proliferation

Yuri Pivovarenko (Research and Training Center “Physical and Chemical Materials Science” Under Kyiv Taras Shevchenko University and NAS of Ukraine, Kiev, Ukraine)

Article ID: 2742

DOI: https://doi.org/10.30564/jor.v3i1.2742


It was previously found that the electric charge of water determines its ability to interact with other substances, including biologically significant ones. It is shown here that the electric charge of water can also determine its ability to penetrate and accumulate in living cells. In particular, it has been shown that the high penetrating ability of positively charged water determines both its active penetration into cells and accumulation in them, which creates favourable conditions for cell proliferation. At the same time, it has been shown that the low penetrating ability of negatively charged water determines its ability to slow down cell  proliferation. It also discusses how medics can obtain and use water at different charges.


Water;Cell proliferation;DNA oxidation;Cancer;Photodynamic therapy;Hyperthermia

Full Text:



[1] Pivovarenko Y. The Electric Potential of the Female Body Liquids and the Effectiveness of Cloning.Research and Reviews on Healthcare: Open Access Journal, Lupine Publishers, LLC, 2018, 1(2): 22-26.

[2] Pivovarenko Y. ±Water: Demonstration of Water Properties, Depending on its Electrical Potential.World Journal of Applied Physics, 2018, 3(1): 13-18.DOI: 10.11648/j.wjap.20180301.12


[4] Pivovarenko Y. Arborization of Aqueous Chlorides in Pulsed Electromagnetic Fields as a Justification of Their Ability to Initiate the Formation of New Neuronal Dendrites. International Journal of Neurologic Physical Therapy, 2019, 5(1): 21-24.DOI: 10.11648/j.ijnpt.20190501.14

[5] Goodwin T.J. Physiological and Molecular Genetic Effects of Time-Varying Electromagnetic Fields on Human Neuronal Cells; Technical Report of NASA.Lyndon B. Johnson Space Center Houston, Texas,2003: 30.

[6] Reardon S. Performance boost paves way for “brain doping”. Nature, 2016, 531: 283-284.

[7] Pivovarenko Y. The Value of Gaseous Hydrogen Generated by the Intestinal Microflora of Human.Chapter 07 in: Top 10 Contributions on Biomedical Sciences: 2nd Edition, 2018: 2-15.

[8] Pivovarenko Y. Negative Electrization of the Sargasso Sea as the Cause of Its Anomaly. American Journal of Electromagnetics and Applications, 2020, 8(2):33-39.DOI: 10.11648/j.ajea.20200802.11

[9] Reiss F.F. Memoires de la Societe Imperiale des Naturalistes de Moscou, 1809, 2: 327-337.

[10] Mahmoud A., Olivier J., Vaxelaire J. at al. Electrical field: A historical review of its application and contributions in wastewater sludge dewatering. Water Research, 2010, 44: 2381-2407.

[11] Spangenberg J.E., Vennemann T.W. The stable hydrogen and oxygen isotope variation of water stored in polyethylene terephthalate (PET) bottles. Rapid Communications in Mass Spectrometry, 2008,22:672-676.


[13] Lane N. Why Are Cells Powered by Proton Gradients? Nature Education, 2010, 3(9): 18.


[15] Lane N., Allen J.F. and Martin W. How did LUCA make a living? Chemiosmosis in the origin of life. Bioassays, 2010, 32: 271-280.

[16] Beyenbach K.W. Transport of magnesium across biological membranes. Magnesium and Trace Elements,1990, 9(5): 233-254.

[17] Taiz L., Zeiger E. Plant Physiology, 3rd ed. Sunderland (UK): Sinauer Associates, Inc, 2002:623р.

[18] Lysak V.V. Microbiology. Minsk: Publishing House of Belarus State University, 2007: 426. (in Russian)

[19] Zhao Y., Quick M., Shi L., Mehler E.L., Weinstein H.,Javitch J.A. Substrate-dependent proton antiport in neurotransmitter: sodium symporters. Nature Chemical Biology, 2010, 6: 109-116.

[20] Hata M., Miyanaga N., Koichi T. at al. Proton beam therapy for invasive bladder cancer: a prospective study of bladder-preserving therapy with combined radiotherapy and intra-arterial chemotherapy. International journal of radiation oncology, biology, physics, 2006, 64(5): 1371-1379.DOI: 10.1016/j.ijrobp.2005.10.023

[21] Nichols R.C., Huh S., Li Z. and Rutenberg M. Proton therapy for pancreatic cancer. World Journal of Gastrointestinal Oncology, 2015, 7(9): 141-147.

[22] Saira E.A., Brooks E.D., Holliday E.B. Proton therapy for colorectal cancer. Applied Radiation Oncology, 2019: 17-22.


[24] Afanasyev V., Korol B., Mantsygin Y. Two forms of cell death: cytometric and biochemical analysis.Reports of the USSR Academy of Sciences, 1985,285(2): 451-455.

[25] Day R.M. and Suzuki Y.J. Cell Proliferation, Reactive Oxygen and Cellular Glutathione. Dose response, 2005, 3(3): 425-442.DOI: 10.2203/dose-response.003.03.010

[26] Nekrasov B. V. General chemistry, 1. Moscow:Chemistry, 1974: 656. (in Russian).

[27] Maloney P.C., Kashket E.R., Wilson T.H. A Protonmotive Force Drives ATP Synthesis in Bacteria.PNAS, 1974, 71(10): 3896-3900.

[28] Farha M.A., Verschoor C.P., Bowdish D., Brown W.D. Synergy by Collapsing the Proton Motive Force. Chemistry & Biology, 2013, 20: 1168-1178.

[29] Efremov R.G., Baradaran R. and Sazanov L. The architecture of respiratory complex I, Nature,2010, 465: 441-445.

[30] Pivovarenko Y. The Electric Potential of the Tissue Fluids of Living Organisms as a Possible Epigenetic Factor. Chemical and Biomolecular Engineering,2017, 2(3): 159-164.DOI:10.11648/j.cbe.20170203.15

[31] Pivovarenko Y. Influence of Glass and Air on Our Perception of DNA. European Journal of Biophysics,2020, 8(1): 10-15.DOI: 10.11648/j.ejb.20200801.12

[32] Kursar T. and Holzwarth G. Backbone Conformational Change in the A-B Transition of Deoxyribonucleic Acid. Biochemistry, 1976, 15(15): 3352-3357.

[33] Saenger W. Principles of Nucleic Acid Structure.New York - Berlin - Heidelberg - Tokyo:Springer-Verlag, 1984: 556.

[34] Leal C., Wadso L., Olofsson G., Miguel M., Wennerstro H. The Hydration of a DNA-Amphiphile Complex. Journal of Physical Chemistry. B, 2004, 108:3044-3050.


[36] Terentyeva Y., Pivovarenko Y. UV Absorbance of lymphocytes. European Journal of Advanced Research in Biological and Life Sciences, 2015, 3(4):20-24.

[37] Pivovarenko Y. UV Absorbance of Aqueous DNA.European Journal of Biophysics, 2015, 3(3):19-22.DOI: 10.11648/j.ejb.20150303.11

[38] Doshi R., Day P.J.R., Tirelli N. Dissolved oxygen alteration of the spectrophotometric analysis and quantification of nucleic acid solutions. Biochemical Society Transactions, 2009, 37: 466-470.

[39] Doshi at al. Spectrophotometric analysis of nucleic acids: oxygenation-dependant hyperchromism of DNA. Anal. Bioanal. Chem., 2010, 396: 2331-2339.

[40] Frenkel K. Carcinogen-mediated oxidant formation and oxidative DNA damage. Pharmacology & Thera peutics, 1992, 53: 127-166.

[41] Malins D.C. Precancerous diagnostics for breast cancer by studying free-radical damage to DNA.Optical Engineering Reports, 1995, 135: 1-3.

[42] Poli G., Parola M. Oxidative damage and fibrogenesis. Free Radical Biology & Medicine, 1997,22:287-305.

[43] Poulsen H.E., Prieme H., Loft S. Role of oxidative DNA damage in cancer initiation and promotion. European Journal of Cancer Prevention, 1998, 7: 9-16.

[44] Khan F. and Ali R. Enhanced recognition of hydroxyl radical modified plasmid DNA by circulating cancer antibodies. Journal of Experimental & Clinical Cancer Research, 2005, 24: 289-296.


[46] Pivovarenko Y. An Alternative Strategy in Cancer Chemotherapy, Aimed Not at Killing Cancer Cells,but the Recovery of Their DNA, Modified by Active Oxygen. Biomedical Sciences, 2017, 3(5), 94-98.DOI: 10.11648/j.bs.20170305.12

[47] Babbs C.F., Griffin D.W. Scatchard analysis of methane sulfinic acid production from dimethyl sulfoxide:a method to quantify hydroxyl radical formation in physiological systems. Free Radical Biology & Medicine, 1989, 6: 493-503.

[48] Ackroyd R., Kelty C., Brown N. and Reed M. The history of photodetection and photodynamic therapy.Photochemistry and Photobiology, 2001, 74: 656-669.DOI: PubMed

[49] Agostinis P., Buytaert E., Breyssens H. and Hendrickx N. Regulatory pathways in photodynamic therapy induced apoptosis. Photochemical and Photobiological Sciences, 2004, 3: 721-9.DOI: PubMed

[50] Bacellar I.O.L., Tsubone T.M., Pavani C., Baptista M.S. Photodynamic efficiency: from molecular photochemistry to cell death. International Journal of Molecular Sciences, 2015, 16: 20523-20559.DOI:PubMed PMC


[52] Dos Santos A.F., de Almeida D.R.Q., Terra L.F., Baptista M.S., Labriola L. Photodynamic therapy in cancer treatment - an update review. Journal of Cancer Metastasis and Treatment, 2019, 5:25.DOI: 10.20517/2394-4722.2018.83

[53] Van der Zee J. Heating the patient: a promising approach? Annals of Oncology, 2002, 13(8): 1173-1184.DOI: PubMed Abstract

[54] Hildebrandt B., Wust P., Ahlers O., et al. The cellular and molecular basis of hyperthermia. Critical Reviews in Oncology/Hematology, 2002, 43(1): 33-56.DOI: PubMed Abstract

[55] Crawford F. Waves in BPC, 3. Moscow: Nauka, 1974: 528. (in Russian)

[56] Pivovarenko Y. The Use of Electromagnetic Forces of the Earth in Manual and Physiotherapy.Journal of Human Physiology, 2020, 2(1): 10-15.

[57] Kuznetsov V.V., Cherneva N.I. and Druzhin G.I. On the Influence of Cyclones on the Atmospheric Electric Field of Kamchatka. Reports of the Academy of Sciences, 2007, 412(4): 1-5.


  • There are currently no refbacks.
Copyright © 2021 Author(s)

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.